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Destructive biomass sampling systems to estimate tree growth and nutrient use. Neutron probe and sap flow technologies will be employed to obtain comparative water use data. Soil nutrients, root, Rhizobium and mycorrhizal distributions will be assessed by systematic soil coring and litter inputs by littertrap and litter bag decomposition techniques.
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Characterisation of bacterial strains by, PCR, RFLP and RAPD, stress tolerance of strains will be assessed under controlled conditions in the laboratory, the effects of varying light on N2 fixation and water use efficiency will be assessed by porometry, d15N and d13C in controlled conditions.
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Tree growth stimulation by microsymbionts will be assessed by inoculation of nursery stock and subsequent outplanting. Comparative growth stimulation by native and exotic Rhizobial strains will be assessed after outplanting in the field. Molecular methods will be utilised to assess whether inoculated strains of Rhizobium persist on seedlings after transplanting.